CCL2 anticorps

N° du produit ABIN964751

N° du produit (Fournisseur): 209-401-b60

L’anticorps Lapin Polyclonal anti-CCL2 a été validé pour WB et ELISA. Il convient pour détecter CCL2 dans des échantillons de Humain.

Aperçu rapide pour CCL2 anticorps (ABIN964751)

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL2 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-CCL2 anticorps

N° du produit (Fournisseur): 209-401-b60

Fournisseur: Rockland

Fonction: MCP-1 Antibody

Réactivité croisée (Details): This antibody is specific for human MCP-1 protein.

Attributs du produit: Synonyms: rabbit anti-MCP-1 Antibody, CCL2, C-C motif chemokine 2, Monocyte Chemotactic and Activating Factor, MCAF, Small-inducible cytokine A2, SCYA2, Monocyte chemotactic protein 1, Monocyte secretory protein JE, HC11

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E.coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11,025 Da in size corresponding to the mature human MCP-1 protein by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 6 months

Détails sur CCL2

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: CCL2

Sujet: Background: MCP-1, or Monocyte chemoattractant protein 1, of the intercrine beta (chemokine CC) family, is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. It augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. MCP-1 exists as a monomer or homodimer, in equilibrium. It binds to CCR2 and CCR4 and is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

ID gène: 6347

NCBI Accession: NP_002973

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2